Table 1.
Whole secretome proteins identified in common from KS1 and ΔAbpA.
| Protein/Gene Name | Gene Index | Predicted Function/Domain | Mol. Wt. (kDa) |
Pep. Id. |
Aa. Seq. Cov. (%) |
|---|---|---|---|---|---|
| General stress protein gsp-781* | SGO_2107 | Unknown | 41 | 211 | 63 |
| Glucosyltransferase G gtfG* | SGO_0497 | Glycosylhydrolase | 178 | 175 | 62.8 |
| Serine protease challisin sgc* | SGO_0566 | Serine protease | 165 | 131 | 52.2 |
| Serine protease degP* | SGO_2150 | Serine protease | 42 | 102 | 43.6 |
| Surface-associated protein cshA* | SGO_0854 | Unknown | 264 | 85 | 40.1 |
| Streptococcal surface protein A sspA* | SGO_0210 | Unknown | 172 | 75 | 38.3 |
| Surface antigen SCP-like domain | SGO_1110 | Unknown | 82 | 72 | 46.2 |
| Membrane protein | SGO_0060 | Membrane protein | 109 | 66 | 41.8 |
| 5′-nucleotidase family protein* | SGO_1247 | N-terminal metallophosphatase domain | 78 | 64 | 55.6 |
| Streptococcal surface protein B sspB* | SGO_0211 | Unknown | 164 | 58 | 38.7 |
| LPXTG cell wall surface protein* | SGO_0707 | Cell wall surface protein | 180 | 52 | 54.6 |
| LPXTG cell wall surface protein* | SGO_1651 | Cell wall surface protein | 84 | 50 | 37.9 |
| Surface-associated protein cshB* | SGO_1148 | Unknown | 244 | 46 | 32.9 |
| Penicillin-binding protein 1B pbp1B | SGO_1928 | Transpeptidase domain | 92 | 34 | 43.6 |
| Exo-beta-D-fructosidase* | SGO_0385 | Glycosyl hydrolases family 32 | 156 | 32 | 29.9 |
| LPXTG cell wall surface protein* | SGO_0890 | Collagen binding domain | 69 | 32 | 22.7 |
| Zinc metalloproteinase B zmpB* | SGO_0408 | IgA1-specific Metallo-endopeptidase N-terminal region |
218 | 28 | 18.2 |
| Putative transcriptional regulator lytR* ∞ | SGO_0535 | Membrane bound transcriptional regulator | 44 | 28 | 44.2 |
| Lipoprotein* | SGO_1082 | Lipoprotein | 37 | 28 | 70.2 |
| LPXTG cell wall surface protein* | SGO_1487 | Cna protein B-type domain | 191 | 28 | 50.7 |
| Putative N-acetylmuramidase* | SGO_2013 | Glycosyl hydrolases family 25 | 127 | 28 | 33.4 |
| Lipoprotein* | SGO_0094 | Unknown | 42 | 26 | 43.1 |
| Putative uncharacterized protein | SGO_1066 | Unknown | 49 | 26 | 53.9 |
| Conserved domain protein* | SGO_0021 | Unknown | 20 | 25 | 60.4 |
| Putative uncharacterized protein* | SGO_2136 | Unknown | 48 | 25 | 47.2 |
| Putative uncharacterized protein | SGO_0319 | Unknown | 32 | 23 | 26.1 |
| LysM domain protein* | SGO_0138 | Involved in binding peptidoglycan | 40 | 21 | 17.7 |
| Oligopeptide-binding protein* | SGO_1716 | ABC-type oligopeptide transport system | 73 | 20 | 35.7 |
| LPXTG cell wall surface protein* | SGO_0388 | Zinc carboxypeptidase | 120 | 19 | 22.3 |
| LPXTG cell wall surface protein* | SGO_0430 | Unknown | 96 | 19 | 35.3 |
| Serine/threonine protein kinase | SGO_0600 | Serine/threonine protein kinase | 67 | 18 | 24.6 |
| Maltose/maltodextrin-binding protein* | SGO_0104 | Maltose binding domain | 45 | 17 | 44.2 |
| Oligopeptide-binding lipoprotein hppH* | SGO_1715 | ABC-type oligopeptide transport system | 72 | 17 | 32.2 |
| Protein with prophage function domain | SGO_0067 | Prophage function domain | 52 | 16 | 35 |
| Glyceraldehyde-3-phosphate dehydrogenase gap |
SGO_0207 | Glycolysis | 36 | 15 | 41.1 |
| Transport protein | SGO_0767 | TroA like transporter domain | 32 | 14 | 47.8 |
| LPXTG cell wall surface protein* | SGO_1650 | Collagen binding domain | 77 | 14 | 18.7 |
| Pneumococcal histidine triad A protein | SGO_1313 | Unknown | 124 | 13 | 13 |
| Autolysin, N-acetylmuramidase-like protein | SGO_1138 | Unknown | 26 | 12 | 40.2 |
| Sulfatase* | SGO_1377 | Sulfatase | 83 | 12 | 26.3 |
| LPXTG cell wall surface protein | SGO_2005 | Unknown | 397 | 12 | 4.9 |
| LPXTG cell wall surface protein* | SGO_0107 | Collagen binding domain | 115 | 11 | 10.9 |
| Amylase-binding protein B abpB* | SGO_0162 | Amylase-binding protein | 73 | 11 | 25.5 |
| Lipoprotein* | SGO_0181 | Unknown | 36 | 11 | 36.7 |
| Cell wall binding protein* | SGO_0477 | Glucan-binding domain | 36 | 11 | 42.4 |
| Peptidoglycan N-acetylglucosaminedeacetylase A pgdA* |
SGO_0948 | Peptidoglycan N-acetylglucosaminedeacetylase |
54 | 11 | 21.1 |
| Penicillin-binding protein 2B pbp2B | SGO_1449 | Transpeptidase domain | 75 | 11 | 22.8 |
| Oligopeptide-binding lipoprotein hppG* | SGO_1713 | ABC-type oligopeptide transport | 74 | 11 | 28.5 |
| Lipoprotein* | SGO_0068 | Unknown | 20 | 10 | 42.4 |
| Lipoprotein hlpA* | SGO_0458 | ABC-type metal ion transport system | 32 | 9 | 29.6 |
| Cell wall binding protein* | SGO_0478 | Glucan-binding domain | 37 | 9 | 38 |
| Substrate-binding protein msmE* | SGO_1305 | Sugar transport | 49 | 9 | 35.9 |
| Branched-chain amino acid ABC transporter* | SGO_1630 | Amino acid-binding protein | 41 | 9 | 25.3 |
| Conserved domain protein* | SGO_0018 | Transglycosylase-like domain | 21 | 8 | 25.5 |
| Elongation factor Tu tuf | SGO_0761 | Protein translation | 44 | 7 | 23.4 |
| Enolase eno | SGO_1426 | Glycolysis | 47 | 7 | 17.1 |
| Cell wall binding protein* | SGO_0845 | Glucan-binding domain | 34 | 7 | 12.8 |
| Cell shape-determining protein mreC* | SGO_2108 | Rod shape-determining protein | 31 | 7 | 29.6 |
| Penicillin-binding protein 1A pbp1a* | SGO_0586 | Penicillin binding protein transpeptidase domain |
79 | 6 | 11.7 |
| Putative uncharacterized protein | SGO_0591 | Unknown | 50 | 6 | 10.5 |
| SCP-like extracellular protein* | SGO_0847 | SCP-like extracellular protein domain | 56 | 6 | 14.9 |
| Putative uncharacterized protein | SGO_0332 | Unknown | 53 | 5 | 15.6 |
| Amino acid ABC transporter* | SGO_0982 | Amino acid-binding protein domain | 31 | 5 | 18.7 |
| Foldase protein prsA* | SGO_1572 | PPIase domain | 34 | 4 | 18.8 |
| Penicillin-binding protein 2× pbp2×* | SGO_0575 | Penicillin binding protein transpeptidase domain | 83 | 4 | 5.7 |
| Efflux transporter, RND family, MFP subunit family* |
SGO_0750 | RND family efflux transporter | 42 | 4 | 18.3 |
| Lipoprotein* | SGO_1165 | Unknown | 33 | 4 | 21.6 |
| Sortase A srtA* | SGO_1230 | Cell wall anchoring of LPXTG proteins (Nobbs, 2007) |
28 | 4 | 8.8 |
| LPXTG cell wall surface protein* | SGO_1415 | X-prolyldipeptidylaminopeptidase | 117 | 4 | 7.6 |
| Peptidyl-prolylcis-trans isomerase* | SGO_1463 | Peptidyl-prolylcis-trans isomerase | 29 | 4 | 12.7 |
| Metal ABC transporter substrate-binding lipoprotein* |
SGO_1802 | Metal ABC transporter | 35 | 4 | 22.6 |
| Elongation factor G fusA | SGO_0206 | Protein translation | 77 | 3 | 10.2 |
| Chaperone protein dnaK | SGO_0402 | Protein folding | 65 | 3 | 4.9 |
| L-lactate dehydrogenase ldh | SGO_1232 | L-lactate dehydrogenase | 35 | 3 | 9.1 |
| Putative lipoprotein* | SGO_0004 | Unknown | 20 | 3 | 17.4 |
| Possible cell wall protein* | SGO_0846 | SCP-like extracellular protein domain n | 76 | 3 | 5.2 |
| Glutamine ABC transporter permease and substrate binding protein* |
SGO_1037 | ABC transporter | 53 | 3 | 6.4 |
| Thioredoxin family protein | SGO_1171 | Thioredoxin | 18 | 3 | 31.2 |
| D-Alanyl-D-Alanine carboxypeptidase* | SGO_1585 | D-alanyl-D-alanine carboxypeptidase | 51 | 3 | 6.7 |
| 5′-nucleotidase, lipoprotein* | SGO_1860 | Haloaciddehalogenase-like hydrolases | 32 | 3 | 11.2 |
| Putative uncharacterized protein | SGO_1870 | Unknown | 65 | 3 | 5.1 |
| Putative uncharacterized protein* | SGO_1932 | Unknown | 22 | 3 | 24.6 |
| Lipoprotein* | SGO_2038 | Unknown | 17 | 3 | 21.4 |
| Lipoprotein* | SGO_0227 | Unknown | 23 | 3 | 8.1 |
| Arginine–tRNA ligase argS | SGO_2058 | Arginine–tRNA ligase | 63 | 3 | 6.9 |
| Putative peptidoglycan hydrolase and general stress protein* |
SGO_0212 | Putative peptidoglycan hydrolase | 23 | 3 | 12 |
| Putative uncharacterized protein pXO1 | SGO_0059 | Unknown | 11 | 2 | 35.8 |
| Putative uncharacterized protein | SGO_0080 | Unknown | 62 | 2 | 5.3 |
| Putative acyltransferase* | SGO_0112 | Putative acyltransferase | 68 | 2 | 3.5 |
| Putative uncharacterized protein* | SGO_0329 | Unknown | 69 | 2 | 5.4 |
| Aminodeoxychorismatelyase-like protein | SGO_0518 | Unknown | 60 | 2 | 3.3 |
| Pyruvate kinase pyk | SGO_1339 | Glycolysis | 55 | 2 | 7.4 |
| ABC transporter, substrate-binding protein* | SGO_1763 | ABC transporter | 54 | 2 | 7.6 |
| LPXTG cell wall surface protein, glycosyl hydrolase* |
SGO_0208 | Glycosyl hydrolase family 85 | 174 | 2 | 1.6 |
| Lipoprotein* | SGO_1931 | Unknown | 19 | 2 | 9.4 |
| ABC transporter, substrate-binding protein* | SGO_0457 | ABC transporter | 32 | 2 | 6.7 |
| Putative uncharacterized protein | SGO_0832 | Unknown | 13 | 2 | 26.3 |
| Penicillin-binding protein 2A pbp2A | SGO_2010 | Penicillin binding protein transpeptidase domain |
81 | 2 | 2.6 |
| Penicillin-binding protein 3 pbp3* | SGO_1717 | Penicillin-binding protein domain | 46 | 2 | 5.6 |
| Elongation factor Ts tsf | SGO_2000 | Protein translation | 37 | 2 | 7.2 |
| Putative uncharacterized protein | SGO_1756 | Unknown | 19 | 2 | 16.1 |
| Zinc proteinase* | SGO_2009 | Zinc metalloprotease | 34 | 2 | 7.9 |
Mol. Wt. kDa – Molecular weight in kDa (http://web.expasy.org/compute_pi/)
Pep. Id. – No. of peptides identified in MS/MS analysis
Aa. Seq. Cov. – % of the total aminoacid sequence covered by identified peptides
*
Proteins with N-terminal signal for secretion (http://www.predisi.de/home.html)