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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Drug Alcohol Depend. 2014 Dec 18;0:1–19. doi: 10.1016/j.drugalcdep.2014.12.005

Figure 5.

Figure 5

Different effects on the cysteine accessibility of DAT T316C/C306A induced by various inhibitors. (A) Hong et al., unpublished results. (B) Adapted from Hiranita et al, 2014. HEK293 cells stably transfected with T316C/C306A human DAT were labeled with maleimide-PEO2-biotin in the presence of DAT inhibitors. Biotinylated DAT proteins were enriched with avidin beads from cell lysates and detected with DAT-specific antibodies. Mean densities of DAT bands were quantified using the NIH ImageJ software and normalized to percent of vehicle (error bar is SEM). Representative blot from n = 3 - 6 experiments. Quantified DAT band densities were analyzed by one way ANOVA with post hoc Dunnett's test or Fisher's least significant difference test. **, P<0.01, *P<0.05 compared with vehicle. See method details in Hong and Amara (2010) and (Hiranita et al., 2014).