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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: J Neuroimmunol. 2014 Nov 26;0:289–298. doi: 10.1016/j.jneuroim.2014.11.020

Figure 2.

Figure 2

Sex differences in peripheral immune frequencies following stroke are CD4+ T cell based. Blood was harvested 96 hours after MCAO from spleen-intact and splenectomized mice and immunophenotyped by flow cytometry. The frequency of CD3+ T cells was determined (A). CD4:CD8 ratio was determined by dividing the frequency of CD4+ cells by the frequency of CD8+ cells (B). CD4 and CD8 T cells were gated on CD3 and the frequency was reported as the percent CD4 or CD8 positive of total CD3+ cells (C,D). Values represent mean numbers (±SEM) of 5 mice from each group for CD4 and CD8 analysis and 8–9 mice per group for CD3 analysis. * indicates p<0.05, ** indicates p <0.01 and *** indicates p<0.001 by t-test.