Figure 1.
CLP-induced lymphocyte lymphopenia is C5a receptor-dependent. A) Blood leukocyte numbers 3 days after CLP in Wt mice or Wt, C5aR1−/−, and C5aR2−/− mice (n=5–10 mice per group). B) Splenic leukocyte numbers 3 days after CLP in Wt mice or Wt, C5aR1−/−, and C5aR2−/− mice (n=5 mice per group). C) Representative TUNEL labeling of spleen sections from Wt or C5aR1−/− mice 20 hrs after CLP (or sham Wt). The scale bar is for all images. D) Aggregate data from TUNEL labeling expressed as the number of TUNEL+ cells per microscopic field (n=5 mice per group).