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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Immunol. 2014 Dec 29;194(3):1285–1291. doi: 10.4049/jimmunol.1402354

Figure 3. The effect of C3 deficiency on inflammatory cell infiltration and vascularization in the wound area.

Figure 3

Representative histological images from one of at least three animals per C3−/− and C3+/+ group are shown. A, B, C) H&E-stained wound cross-sections showing leukocyte infiltration in intramuscular connective tissue bands (open arrows) underlying the ulcer bed (asterisks) (×400; A, B), with quantification of leukocyte infiltrate (C); n=4 mice each. D, E, F) Naphthol AS-D Chloroacetate-esterase(+) mast cells (arrows) in the dermis of unwounded skin, adjacent to wounded edge (×400; D, E), with quantification of mast cells in the wounded areas of the mice (F); n=4 and n=6 mice each. G, H, I) Immunohistochemical demonstration of vessels in the hypodermis (white adipose tissue area between dermis [d] and panniculus carnosus muscle [m]), from sections stained with a monoclonal antibody against alpha-smooth muscle actin (a-SMA) (×400; G, H), with quantification of vessels in the wounded areas of the mice (I); n=3 and n=6 mice each.