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ATX and LPA receptor profiling in (A) B16F10 and (B) mouse lung vascular endothelial cells (MLVEC) using quantitative real-time PCR. Time course of ATX activity measured in the conditioned media (CM) of (C) B16F10 and (D) MLVEC. Control represents media that were not exposed to cells. Data are expressed as mean ± SD of an experiment performed twice in quadruplicates (for quantitative real-time PCR) and three times in triplicates for the ATX activity assay.
