Fig. 4.

SLC4A11-C and mutant R109H mediated H⁺(OH⁻) flux following an acute decrease in pH_i. NH₄⁺ removal experiments were done under chemically voltage-clamped conditions with 110 mM K⁺- and valinomycin-containing solutions in the presence or absence of Na⁺ and Cl⁻. pH_i was decreased following NH₄⁺ removal to ∼6.7, and the H⁺(OH⁻) flux was determined during the initial phase of pH_i recovery. A: Na⁺- and Cl⁻-containing solutions: SLC4A11-C-expressing cells; B: Na⁺-free Cl⁻-containing solutions: SLC4A11-C-expressing cells; C: Na⁺-free Cl⁻-free solutions: SLC4A11-C-expressing cells; D: Na⁺- and Cl⁻-containing solutions: mock-transfected cells; E: Na⁺-free Cl⁻-containing solutions: mock-transfected cells; F: Na⁺-free Cl⁻-containing solutions: R109H mutant-expressing cells.