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. 2014 Nov 12;308(2):C176–C188. doi: 10.1152/ajpcell.00271.2014

Fig. 7.

Fig. 7.

Modulation of SLC4A11-C and mutant R109H H+(OH−) flux by 4,4′-diisothiocyano-2,2′-stilbenedisulfonate (DIDS) during changes in extracellular pH. The cells were preincubated with DIDS in the cell culture incubator for 1 h at 37°C before initiating the experiments in DIDS-free solutions. During the experiments, the cells were chemically voltage clamped with 110 mM K+-valinomycin. A: mock-transfected cells with DIDS preincubation; B: SLC4A11-C-expressing cells with DIDS preincubation; C: R109H-expressing cells with DIDS preincubation. D: dose dependence of DIDS stimulation of SLC4A11-C H+(OH−) flux. The cells were preincubated with various DIDS concentrations (0–1,000 μM). SLC4A11-C H+(OH−) flux was measured during the change of extracellular pH from 7.4 to 6.2 while the cells were chemically voltage clamped with 110 mM K+-valinomycin.