Table 2.

Effect of DIDS on H⁺(OH⁻) flux

	Mock	SLC4A11-C	R109H	DIDS Mock	DIDS SLC4A11-C	DIDS R109H
	Effect of DIDS on H+(OH−) Flux: pH 7.4 → pH 6.2
Initial pHi	7.02 ± 0.05	7.05 ± 0.043	7.05 ± 0.03	7.05 ± 0.03	7.21 ± 0.06	7.04 ± 0.03
Minimum pHi	6.88 ± 0.031	6.67 ± 0.043,4	6.90 ± 0.017	7.00 ± 0.03	6.51 ± 0.04	6.69 ± 0.03
ΔpHi	0.15 ± 0.031	0.38 ± 0.034,5	0.15 ± 0.038	0.06 ± 0.02	0.69 ± 0.02	0.35 ± 0.03
Initial Hin+, nM	95.9 ± 10.0	91.1 ± 7.173	89.2 ± 5.66	89.3 ± 6.44	64.1 ± 8.93	93.3 ± 5.73
Minimum Hin+, nM	133.4 ± 9.001	218.5 ± 17.94,6	125.4 ± 4.088	101.8 ± 6.96	310.6 ± 26.6	209 ± 12.9
ΔHin+, nM	37.6 ± 5.042	127.4 ± 13.64,5	36.2 ± 6.588	12.5 ± 5.07	246.6 ± 18.4	116 ± 12.6
H+(OH−) flux, μM/s	23.4 ± 5.96	62.4 ± 4.914,5	31.1 ± 3.159	23.8 ± 2.03	154.6 ± 7.47	43.4 ± 2.14
n	3	6	4	4	4	11
	Effect of DIDS on H+(OH−) Flux: pH 6.2 → pH 7.4
Final pHi	6.93 ± 0.042	6.99 ± 0.0310	6.99 ± 0.03	7.08 ± 0.02	7.13 ± 0.03	6.95 ± 0.02
ΔpHi	0.05 ± 0.01	0.32 ± 0.024,5	0.09 ± 0.017	0.08 ± 0.01	0.62 ± 0.02	0.27 ± 0.02
Final Hin+, nM	118.3 ± 9.912	103.3 ± 6.1810	102.8 ± 5.85	83.6 ± 4.85	74.3 ± 5.23	112.9 ± 5.77
ΔHin+, nM	15.1 ± 0.92	115.2 ± 12.34,5	22.5 ± 2.338	18.3 ± 3.15	236.4 ± 22.1	96.1 ± 11.0
H+(OH−) flux, μM/s	15.9 ± 0.67	36.2 ± 1.524,5	18.3 ± 2.687	14.0 ± 0.88	107.4 ± 4.61	34.1 ± 1.48
n	3	6	4	4	4	11

n, No. of experiments. DIDS, 4,4′-diisothiocyano-2,2′-stilbenedisulfonate. Note: the absolute values of the delta (Δ) and flux measurements are depicted. ¹P < 0.05 vs. mock + DIDS. ²P < 0.02 vs. mock + DIDS. ³P < 0.05 vs. SLC4A11-C + DIDS. ⁴P < 0.01, SLC4A11-C vs. mock or R109H. ⁵P < 0.001 vs. SLC4A11-C + DIDS. ⁶P < 0.02 vs. SLC4A11-C + DIDS. ⁷P < 0.001 vs. R109H + DIDS. ⁸P < 0.005 vs. R109H + DIDS. ⁹P < 0.01 vs. R109H + DIDS. ¹⁰P < 0.01 vs. SLC4A11-C + DIDS.