Fig. 3.

Inhibition of SUR2B mRNA expression by MGO and exogenous miR-9a-3p. After transfection with m-9, A10 cells were cultured for 12–24 h. Cells transfected with anti-9 were also treated with 300 μM MGO and cultured under the same conditions. A marked reduction in the relative quantity (RQ) of SUR2B mRNA was found in cells transfected with m-9, to the similar degree as the effect of 300 μM MGO treatment. The effect of MGO was significantly reduced in cells transfected with anti-9. Cells transfected with scrambled RNA (scmiR) were used as control. GAPDH was used for internal normalization. *P < 0.05; **P < 0.01 (n = 4).