Fig. 7.

Luciferase activity and level of expression of luciferase and endogenous RFVT-3 mRNAs in jejunum crypt and villus epithelial cells of the transgenic mice carrying SLC52A3 promoter-luciferase construct. Luciferase activity was determined using luminometer, and data are expressed per milligram of protein (A, i). Real-time PCR analysis was performed using gene-specific primers against luciferase (A, ii) and RFVT-3 (B) from total RNA isolated from mice intestinal crypt and villus epithelial cells. Values are means ± SE from 3 separate animals. *P < 0.01 and **P < 0.05.