Table 1.

Sequence of primers used in PCR to generate 5′-deletion constructs of the SLC52A3 promoter

Position	Sequence (5′-3′)
−3,125 bp	CGACGCGTTTGATCAAATCCTGGTTG
−1,509 bp	CGACGCGTTGCAGCTACAAAGAAGG
−9,66 bp	CGACGCGTAGAAGACTCTGAAGCACAG
−199 bp	CGACGCGTAGTGGCTCCTCCCAG
−101 bp	CGACGCGTGTGAGGGAATTCCGTG
Reverse	CCGCTCGAGCTTCTTCCTTCTAGTACAAAGC

MluI and XhoI restriction sites are used in the forward and reverse primers, respectively, as shown in underlined and bold underlined, respectively. The relative positions of the primers are assigned by setting start of transcription initiation of SLC52A3 as position +1.