Fig. 6.

KCl-regulated ion channels were associated with angiogenesis

To examine whether KCl-mediated angiogenesis is regulated through ion channel activity, cells were treated with 25 mM KCl after NS1619 pre-treatment for 15 minutes. Compared with cells treated with KCl alone, cells incubated with both KCl and NS1619 showed 30% decrease in TSP1 expression. These results show that KCl-mediated ion channel regulation inhibits angiogenesis. Proteins levels were normalized to β-actin, the protein loading control (^a)P<0.05 and ^b)P<0.01). KCI, potassium chloride; TSP1, thrombospondin 1.