Fig. 3.

Loss of intestinal epithelial IR does not impact glucose tolerance, blood insulin, or insulin-like growth factor 1 (IGF1) levels after chow or high-fat diet (HFD) feeding but attenuates HFD-induced increases in circulating cholesterol. A and B: blood glucose levels following intestinal absorption and glucose secretion into the circulation (A) and concentration of blood glucose over time [area under the curve (AUC), B] in IR^fl/fl and VC-IR^Δ/Δ mice measured by oral glucose tolerance test. C–E: fed plasma insulin and IGF1 levels measured by ELISA (C and D) and plasma cholesterol levels assessed by colorimetric cholesterol assay (E) in IR^fl/fl and VC-IR^Δ/Δ mice fed chow or HFD (60% of kilocalories from fat). Values are means ± SE; n ≥ 5. *P ≤ 0.05, HFD vs. chow (by 2-way ANOVA with Sidak's post hoc pair-wise comparisons for main effect of diet).