Fig. 8.
Expression level of p21 in the small intestinal epithelium of Klf4fl/fl and Klf4ΔIS mice. A: expression pattern of p21 in Klf4fl/fl sham mice (a and b) and mice at 6 (c and d), 24 (e and f), and 96 h (g and h) following γ-irradiation. p21 is red, and nuclear staining is blue. B: expression pattern of p21 in Klf4ΔIS sham mice (a and b) and at 6 (c and d), 24 (e and f), and 96 h (g and h) following γ-irradiation. p21 is red, and nuclear staining is blue. C: quantification of the number of p21-positive cells in the crypt (region of Ki67 expression) in both mouse groups. D: graph depicting changes in the ratio in the number of p21-positive cells within the crypt in relation to the total number of cells in the crypt (region of Ki67 expression) in both mouse groups following γ-irradiation. The number of positive p21 cells was averaged from at least 20 crypts per mouse; n = 2, *P < 0.05, **P < 0.01, ***P < 0.001 ± SE.