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. 2014 Nov 20;308(2):G121–G138. doi: 10.1152/ajpgi.00080.2014

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Fig. 9. — A: staining for senescence-associated endogenous β-galactosidase (β-gal) activity in the intestines of Klf4^fl/fl (a and c) and Klf4^ΔIS (b and d) mice. B: quantification of β-gal foci per crypt in Klf4^fl/fl and Klf4^ΔIS mice at sham and 96 h following γ-irradiation. The number of positive β-gal foci per crypt was averaged from at least 20 crypts per mouse. C: analysis of mice intestines at 7–9 days postirradiation of Klf4^fl/fl (a–c) and Klf4^ΔIS (d–f) mice. Atrophied crypts are marked by asterisks. D: quantification of the percentage of atrophied crypts per field in Klf4^fl/fl and Klf4^ΔIS mice at 7–9 days following γ-irradiation. The number of atrophied crypts was averaged from at least 10 fields per mouse. E: p53 staining of Klf4^fl/fl (a and b) and Klf4^ΔIS (c and d) mice. p53 is red, and nuclear staining is blue. F: p21 staining of Klf4^fl/fl (a and b) and Klf4^ΔIS (c and d) mice. p21 is red, and nuclear staining is blue; n = 4, ***P < 0.001 ± SE.