Figure 4.
Gene Expression Profiling of Various Mouse Stem Cell Lines
(A) RT-PCR of pluripotency genes. Note naive marker gene expression by mouse E-cad rESC and wit-rESC lines.
(B) Real-time PCR of primed pluripotency genes in mouse E-cad-rESCs, wit-rESCs, and EpiSCs. Expression levels of pluripotency genes are compared to those in mouse ESCs. Expression values are normalized to Gapdh. Data are represented as mean ± SEM of three independent experiments.
(C) Real-time PCR analysis for naive pluripotency genes in mouse E-cad-rESCs, wit-rESCs, and EpiSCs. Expression levels of pluripotency genes are compared to those in mouse ESCs. Relative expression values are normalized to Gapdh.
Data represent mean ± SEM of three independent experiments, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. Note that expression patterns in mouse E-cad-rESCs and wit-rESCs resemble those in mouse ESCs, but not those in mouse EpiSCs.