Figure 4.

SORE6⁺ Cells Are Relatively Resistant to Chemotherapeutics

(A) Cultures of MCF10Ca1h cells after 2 days of treatment with doxorubicin (50 nM) or paclitaxel (25 nM) showing selective killing of SORE6⁻ cells. See also Movies S2 and S3. Scale bar, 200 μm.

(B) Effect of treatment with doxorubicin (Dox; 50 nM) or paclitaxel (Pac; 25 nM) on the relative representation of SORE6⁺ cells in the MCF10Ca1h culture assessed by flow cytometry after 48 hr. Results are mean ± SEM for three technical replicate determinations.

(C) FACS profile of MCF10Ca1h cells after 4 days of treatment with 25 nM or 50 nM paclitaxel (Pac), together with quantitation of SORE6⁺ cells by FACS analysis. Results are mean ± SEM for three technical replicates.

(D) Schematic for treatment of MCF10Ca1h tumors with Cytoxan.

(E) The effect of Cytoxan on SORE6⁺ cell representation in tumors from Cytoxan- or vehicle-treated mice. Results are median ± interquartile range for n = 5–8 mice/group.

See also Movies S2 and S3.