Table 1.
In Vivo Limiting Dilution Assay for MCF10Ca1h Cells
|
Cell Population |
No. of Cells Implanted/Site |
1/CSC Frequency | 95% CI | |||
|---|---|---|---|---|---|---|
| 5,000 |
2,500 |
500 |
100 |
|||
| Tumor Incidence | ||||||
| Sham sort | 5/6 | 4/6 | 1/6 | 1/6 | 2,343 | 4,564–1,203 |
| SORE6− | 2/6 | 1/6 | 0/6 | 0/6 | 14,308 | 44,186–4,633 |
| SORE6+ | 6/6 | 5/6 | 4/6 | 3/6 | 722 | 1,497–438 |
The indicated number of cells was implanted orthotopically into nude mice, and tumor incidence was assessed after 3 months. CSC frequencies were calculated using ELDA software. CI, confidence interval.