Figure 3.

Changes to Ion Channel and Gap Junction Profiles in SHOX2-EBs Favor Automaticity

(A) Immunostaining images of GFP-EB (left) and SHOX2-EB (middle and right) with an antibody against HCN4 channel proteins. DAPI (blue), nuclear staining.

(B) Quantitative measurements of HCN4 (left and middle) and NCX1 (right) protein levels. D6+14 for NCX1.

(C) Transcript (left) and protein (right) level measurements of Cx45 in GFP-EBs and SHOX2-EBs. Protein level was determined with D6+14 samples.

(D) Transcript (left) and protein (right) level measurements of Cx43 in GFP-EBs and SHOX2-EBs. The protein level was determined with D6+14 samples. Data are represented as mean ± SEM. All experiments were performed on three independent biological replicates.

See also Figures S2 and S3.