Table 1.
Compositional details of the semi-continuous D. viridis cultivation medium. All quantities, except for the varying NaCl component (Low Salt: LS, Medium Salt: MS, and High Salt: HS), were identical for each photo-osmotic growth condition
| Medium component | Quantity |
|---|---|
| Artificial seawater | |
| Low Salt (LS): NaCl | 362.7 mM |
| Medium Salt (MS): NaCl | 1.3627 M |
| High Satl (HS): NaCl | 2.3627 M |
| Na2SO4 | 25.00 mM |
| KCl | 8.030 mM |
| NaHCO3 | 2.067 mM |
| KBr | 0.725 mM |
| H3BO3 | 0.433 mM |
| NaF | 0.0657 mM |
| MgCl2·6H2O | 47.18 mM |
| CaCl2·2H2O | 9.134 mM |
| SrCl2·6H2O | 0.0214 mM |
| Nutrients | |
| NaNO3 | 3.00 mM |
| Na2HPO3 | 0.066 mM |
| Trace metals | |
| Na2EDTA·2H2O | 14.86 μM |
| FeCl3·6H2O | 6.562 μM |
| MnSO4·4H2O | 2.42 μM |
| ZnSO4 | 0.245 μM |
| CoSO4 | 56.9 nM |
| Na2MoO4·2H2O | 0.520 μM |
| Na2SeO3 | 1.0 nM |
| Vitamins | |
| Thiamine | 0.297 mM |
| Vitamin B12 | 1.47 μM |
| Biotin | 4.09 μM |
| Soil seawater | 5 % v/v |