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Biofilm in ETEC isolates positive for the lngA gene. From the 62 lngA+ ETEC isolates analyzed, 83.87% were able to form biofilm. Biofilm formation is critical in the pathogenesis of many human bacterial pathogens. ETEC lngA− was used as negative control and its absorbance to classify as weakly biofilm formers (0.129–0.258); moderately biofilm formers (0.259–0.516); and strongly biofilm formers (≥0.517). Enteroaggregative E. coli 042 shows as rhomb on gray (positive control).
