Table 1.
Primer sequences, amplicon size, and melting temperatures used in this study.
| Gene | Primer | Sequence 5′-3′ | Size (bp) | Tm (°C) | References |
|---|---|---|---|---|---|
| lngA | F (J5) | ATG AGC CTG CTG GAA GTT ATC ATT G | 608 | 62 | Mazariego-Espinosa et al., 2010 |
| R (J6) | TTA ACG GCT ACC TAA AGT AAT TGA GTT | ||||
| St | F | ATT TTT CTT TCT GTA TTG TCT T | 190 | 50 | Perez et al., 2010 |
| R | CAC CCG GTA CAA GCA GGA TT | ||||
| Lt | F | GGC CAC AGA TTA TAC CGT GC | 450 | 50 | Levy, 2002 |
| R | CGG TCT CTA TAT TCC CTG TT | ||||
| cfaI | F | GGT GCA ATG GCT CTG ACC ACA | 478 | 55.7 | This study |
| R | AGT AGT ATC TCT TGT AAT GAC | ||||
| cooA (csI) | F | GTC CAC ACC ATC AAC ACC GTT | 320 | 62 | This study |
| R | ATT ATC CTG ACT AAG TCA ACG | ||||
| cs3 | F | GGG CCC ACT CTA ACC AAA GAA | 401 | 55.7 | This study |
| R | TTT AGT TTC AGG TAA TTA CCG | ||||
| cofA (cfaIII) | F | ATC CTT TCG GTT TAT AACAGA ACG G | 713 | 45 | This study |
| R | CGG CTC GCC AAA GTA ATA GAG |
st: gene coding for a heat-stable enterotoxin; lt: gene coding for a heat-labile enterotoxin; lngA: gene coding for the major subunit of longus; cfaI: gene coding for the colonization factor I; cooA: gene coding for the major subunit of CSI; cs3: gene coding for the CS3; cofA: gene coding for the major subunit of CFA/III.