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. Author manuscript; available in PMC: 2016 Jan 2.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Nov 13;456(1):7–12. doi: 10.1016/j.bbrc.2014.11.010

Figure 4. Mdivi-1 enhances death receptor-mediated apoptosis independent of Drp1 and Bax/Bak.

Figure 4

(A) Drp1 wild-type (Drp1+/+) and knockout (Drp1−/−) MEF cells and (C) Bax/Bak wild-type (Bax/Bak+/+) and double knockout (Bax/Bak−/−) MEF cells were treated with increasing doses of Fas ligand alone or with the combination of mdivi-1 (50 µM) for 16 h. Cleavage of caspase-8 and caspase-3 were detected by western blot. The mouse specific anti-caspase-8 antibody recognizes only the cleaved fragment as compared to human specific antibody. (B) Drp1 wild-type (Drp1+/+) and knockout (Drp1−/−) MEF cells and (D) Bax/Bak wild-type (Bax/Bak+/+) and double knockout (Bax/Bak−/−) MEF cells were treated with Fas ligand (300 ng/ml), mdivi-1 (50 µM), or the combination for 16 h. Apoptosis was determined by staining cells with Annexin V and PI. These data represent three independent experiments.