Table 3.
Description of cellular pathways and function of identified hepatic proteins altered O3 inhalation.
| Protein Identification | Accession # | Pathway | Function |
|---|---|---|---|
| Cytochrome b5 | GenBank:AAB67609 | Drug metabolism | Cypb5 mediates the rate of P450 dependent mono-oxygenation reactions through the transfer of the second electron from NADPH. |
| Catechol-O-methyltransferase | GenBank:NP_036663 | Drug metabolism/L-dopa metabolism | COMT enzymatically O-methylates catechol containing compounds. Both over expression and loss of activity has been implicated in vascular diseases as well as neuronal disorders. |
| Galactokinase 1 | GenBank:NP_001008283 | Cell cycle, energy metabolism | First enzyme required for the conversion of galactose to glucose forming galactose-1-phosphate. |
| Actin, beta like 2 | GenBank:NP_001099879 | Cytoskeletal | Important for cell motility, structure, and integrity. |
| Protein disulfide-isomerase | GenBank:NP_037130 | Protein Folding/ER stress | PDI is responsible for inter and intra molecular disulfide bond formation within the endoplasmic reticulum. |
| Tubulin, alpha 1C | GenBank:NP_001011995 | Cytoskeletal | Alpha tubulin is one of two components of tubulin which is assembled to form microtubules. These are important for cell movement and maintenance of shape. |
| Glucose-regulated protein 78 | GenBank:NP_037215 | Protein Folding/ER stress | Located within the ER; GRP78/BiP binds and inactivates the proteins responsible for UPR activation. Also directly binds misfolded proteins to assist in proper folding or clearance. |
| Glycerol kinase | GenBank:NP_077357 | Energy metabolism | Catalyzes formation of glycerol 3-phosphate. Overexpression in H4IIE cells resulted in increased fat storage and alters activity of PPAR-alpha and other transcription factors. |
| Glutathione S-transferase mu 1 | GenBank:AAA41286 | Phase II drug metabolism | Facilitates conjugation of reduced glutathione to electrophilic compounds for increased excretion. |
| D-β-hydroxybutyrate dehydrogenase | GenBank:AAB59684 | Energy/lipid metabolism | Oxidoreductase, mitochondrial. Involved in fatty acid catabolism. BDH1 facilitates formation of the ketone body 3-hydroxybutyrate. |