Fig 1.

Immunoblot of 4-HNE modified proteins in liver mitochondria from control and ethanol fed rats. Mitochondrial protein (100 µg) was separated on a large format 8–20% gradient gel by SDS-PAGE. After electrophoresis, proteins were transferred to nitrocellulose membranes and probed for 4-HNE modifications using the conditions described in the methods section. Panel A: Five major protein bands with molecular weights of approximately 90, 70, 50, 45, and 35 kDa showed strong immunoreactivity for 4-HNE modifications in mitochondria from control (C) and ethanol (E) fed rats. Representative blots are shown for two pairs of control and ethanol fed rats with samples run in duplicate. Panel B: Quantification of the density of the 5 major protein bands showing 4-HNE immunoreactivity in mitochondria from control and ethanol fed rats. Total number of samples run was n=6 pairs of control and ethanol samples.