Table 2.
Identification and quantification of 4-HNE modified proteins in liver mitochondria from control and ethanol-fed rats: results from 2D BN-PAGE.
| Spot no. | Protein | MOWSE | Control | Ethanol | P value |
|---|---|---|---|---|---|
| 1 | Cytochrome c1, Complex III | 142 | 2538±459 | 1654 ± 318 | 0.027 |
| 2 | Dimethylglycine dehydrogenase | 114 | 3828±461 | 5567 ± 480 | 0.013 |
| 3 | Enoyl CoA hydratase | 118 | 3068 | 5577 | − |
| 4 | Cytochrome c | − | 477 | 879 | − |
Protein spot cluster nos. 1–3 were identified by MALDI–TOF as described in the methods section, whereas protein spot no. 4 was identified by immunoblotting. 4-HNE immunoreactivity for enoyl CoA hydratase (spot cluster no. 3, bottom spot) and cytochrome c (spot no. 4) in both control and ethanol samples is reported for two of six pairs of samples run on BN-PAGE gels. Statistics are provided for cytochrome c1 (spot no. 1) and dimethylglycine dehydrogenase (spot cluster no. 2), as these protein spots immunoreactive for 4-HNE were detected in all six pairs of control and ethanol samples. Data represent the mean±SEM for spot cluster densities for 1 and 2, and the mean densities only for spot clusters 3 and 4.