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. 2014 Jan 14;2:156–164. doi: 10.1016/j.redox.2013.12.015

Fig. 2.

Fig. 2

Ion cluster inversion in C94R/C99R eNOS. The upper panel demonstrates that wildtype eNOS contains a Zn4 cluster with four negatively charged sulfur atoms surrounding the Zn2+ cation in a tetrahedral configuration. In the C94R/C99R mutant the introduction of four arginine residue are proposed to stabilize the dimeric interface of eNOS via electrostatic interactions with a PO43 anion. The lower panel shows both the crystal structure of the Zn cluster (PDB ID 3NOS) in wildtype eNOS and computer simulated structure of the C94R/C99R mutant.