Table 1.

Primer sequences for pyrosequencing analysis

DOI: http://dx.doi.org/10.7554/eLife.03416.021

PDCD1 region	Assay	CpG	PCR primers 5′-3′	Sequencing primer 5′-3′	Product length (bp)
CR-C	1	1 and 2	F: AGGTATAAAGGAGGTTTTGTAATAGT	GAGGTTTTGTAATAGTTAGG	186
			R: CCTCAACCACCCAAATTCAAA-BIO
	2	3, 4, 5	F: TGGGTGGTTGAGGTAGTT	GTTGAGGTAGTTGTTAGAT	253
			R: CACCTCACCTCCTACTTATCTCT-BIO
	3	6	F: TGTTTATTTTAGGGTGGTGAGATTTAT	GTTAGGTATTATGTATGTATATAAG	221
			R: TAAAAACCCACCTCACCTCCTACTT-BIO
CR-B	1	8, 9, 10, 11	F: AAAGGAAGAAAAGTTTTAAGAGAAAGTAAG	CTATCCCACATACTCC	167
			R: ACCCAACTATCCCACATACT-BIO
	2	12, 13, 14, 15	F: GGGTTTTTGTTTTTTAGTAGTAAAGGATTA	ATTAAGGTATAGTTTAGGGTA	164
			R: AAAACCAAACTCTTATCCCTTTAAAA-BIO
	3	16 and 17	F: GGTAGGGGAGGGTTTAGT	GTTTTGGGAGTTAAGG	193
			R: TCCTCTCCATTTCTAACCCCTCTTATA-BIO
	4	7	F: AGGGTAGTAGAGTTAGTAAATTTAAGATA	AGTAGAGAAAATAGTGAGAT	103
			R: CTCTTAAAACTTTTCTTCCTTTCAAAATAC-BIO