Table 2.

Primer sequences for bisulfite sequencing and RT-PCR

DOI: http://dx.doi.org/10.7554/eLife.03416.022

Region	Assay	CpG	PCR primers 5′-3′	Product length (bp)
CR-C*		0–4	F: ATAAAGGTATAAAGGAGGTTTTG	363
			R: CCTAACTAACTAATCATTTCT
CR-C (nested)*		1–4	F: GAGGGGATTTTTTTAGTTTTTGT	289
			R: AATTTAATCATTCTACTCTCT
CR-B*		7–14	F: GAAAGGAAGAAAAGTTTTAAG	332
			R: AAACTAAAACCAAACTCTTATCC
CR-B (nested)*		7–14	F: GTTTTTTTGAATTTATAGGGGTG	276
			R: ACTCTTATCCCTTTAAAAAAT
CR-C†	1	3–5	F: AGTTGCCAGATGGTTTCCAG	154
			R: CTGGGGCATTCTGATGATTT
CR-B†	1	10–14	F: CGGGTCCTAGGAAATGTTCA	236
			R: GCCAGACTCTTGTCCCTTTG
	2	7–10	F: TACAGGGGTGTCTGGAGAGG	156
			R: ATGCCCTGAGCTATGCCTTA
GAPDH†			F: CCACTCCCCTTCCCAGTT	147
			R: CCTATAAATACGGACTGC

^*Primer sequences for bisulfite sequencing.

^†Primer sequences for RT-PCR.