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. 2015 Jan 19;5:7846. doi: 10.1038/srep07846

Table 3. Amplification of MP, hMPV and hBoV genes.

Gene   Sequence Size (bp)
MP Forward 5′-CCA ACCAAA CAA CAA CGT TCA-3′ 76
  Reverse 5′-ACC TTG ACTGGA GGC CGT TA-3′  
  Probe 5′-FAM-TCA ACT CGA ATA 'ACG GTG ACTTCT TAC CAC TG-3′-TAMRA  
hMPV Forward 5′-AACCGTGTACTAAGTGATGCACTC-3′ 213
  Reverse 5′-CATTGTTTGACCGGCCCCATAA-3′  
hBoV Forward 5′-TGACATTCAACTACCAACAACCTG-3′ 92
  Reverse 5′-CAGATCCTTTTCCTCCTCCAATAC-3′  
  Probe 5′-FAM-AGCACCACAAAACACCTCAGGGG-3′-TAMRA  

MP, Mycoplasma pneumonia; hMPV, human metapneumovirus; hBoV, human bocavirus; FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.