Table 3. Amplification of MP, hMPV and hBoV genes.

Gene		Sequence	Size (bp)
MP	Forward	5′-CCA ACCAAA CAA CAA CGT TCA-3′	76
	Reverse	5′-ACC TTG ACTGGA GGC CGT TA-3′
	Probe	5′-FAM-TCA ACT CGA ATA 'ACG GTG ACTTCT TAC CAC TG-3′-TAMRA
hMPV	Forward	5′-AACCGTGTACTAAGTGATGCACTC-3′	213
	Reverse	5′-CATTGTTTGACCGGCCCCATAA-3′
hBoV	Forward	5′-TGACATTCAACTACCAACAACCTG-3′	92
	Reverse	5′-CAGATCCTTTTCCTCCTCCAATAC-3′
	Probe	5′-FAM-AGCACCACAAAACACCTCAGGGG-3′-TAMRA

MP, Mycoplasma pneumonia; hMPV, human metapneumovirus; hBoV, human bocavirus; FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.