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. 2014 Dec 3;16(6):484. doi: 10.1186/s13075-014-0484-2

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© Wen et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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UDP-glucose dehydrogenase ( UGDH )-specific siRNA suppressed glycosaminoglycan (GAG) synthesis in human articular chondrocytes. Human articular chondrocytes were treated with UGDH-specific siRNA for 48 h. Then, mRNA and the protein level of UGDH were detected using real-time PCR (A) and western blotting assay (B, C), while GAG content of these chondrocyte cultures were detected with 1,9-dimethylmethylene blue dye (D) and Alcian blue dye (E, F). The protein level of UGDH was presented as gray level of the blots quantified using Quantity One software (Version 4.6, Bio-Rad). Values are presented as mean ± standard error of the mean from at least three independent experiments. *P <0.05 and **P <0.01 versus control.