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. 2014 Dec 3;16(6):484. doi: 10.1186/s13075-014-0484-2

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© Wen et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Gene expressions of trans-regulators of UDP-glucose dehydrogenase ( UGDH ). Specificity protein 1 (Sp1) protein level in was detected in both human and rat cartilage samples (A, B). Relative mRNA levels of Sp1, Sp3 and Krueppel-related zinc finger protein c-Krox (c-Krox) were also detected in human primary chondrocyte treat with IL-1β (C-E). Total and nuclear Sp1 protein level was also detected and quantified (F, G). MNC, microscopically normal cartilage; DC, degenerative cartilage. Scale bars, 100 μm. Values are presented as mean ± standard error of the mean from at least three independent experiments. *P <0.05 and **P <0.01 versus control.