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. 2015 Jan 19;15:6. doi: 10.1186/s12870-014-0369-1

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© Lionetti et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Degree and pattern of pectin methylesterification (DM) in cell wall extracted from spikes of 02-5B-318 _R and Saragolla _S plants. (a) The DM was quantified and expressed as methanol to uronic acid molecular ratio (%). Data represent the average ± standard deviation (n = 6). Asterisks indicate data sets significantly different between 02-5B-318_R and Saragolla_S according to Student’s t-test (p < 0.01). (b) Immunodot analysis of pectin extracted from spikes of 02-5B-318_R and Saragolla_S plants using LM7 antibody. The micrograms of chelating agent soluble solid fractions from the two genotypes applied to the nitrocellulose membrane were indicated. The experiments were repeated three times with similar results. R = 02-5B-318_R; S = Saragolla_S.