FIGURE 3.

A1 knockdown does not affect MLMCs. (A) Representative dot plots confirming expression of CD117 and FcεRI in MLMCs after 4 wk in culture. (B) Number of MLMCs in culture over time. Cells were sensitized with 1 μg/ml of IgE–anti-TNP for 90 min and left treated or subsequently challenged with 100 ng/ml TNP to induce FcεRI cross-linking (FcεRI-XL) or activated with the ionophores ionomycin (0.5 μg/ml) or A23187 (0.5 μM). As a control, the cells were kept in media without cytokines. Release of β-hexosaminidase (C) and change in cellularity (D) were measured 24 h posttreatment. Symbols represent means ± SEM of six MLMC cultures from individual mice/genotype.