FIGURE 5.

IgE-dependent systemic PSA is markedly reduced in A1-knockdown mice. (A) WT mice (n = 12), VVFF mice (n = 8), and VVA1 mice (n = 8) were sensitized passively by i.p. injection of 10 ng of DNP-specific IgE (SPE-7) and then challenged by i.p. injection the next day with 200 μg of DNP. Body temperature was measured immediately before and in 5-min intervals after Ag challenge as a read-out of the allergic response. Symbols represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, versus WT and VVFF mice, two-way ANOVA with Bonferroni correction for multiple comparisons. The data shown were pooled from four independent experiments performed, each of which gave similar results. Serum histamine (B) and MCP-1 (C) and MCP-2 (D) concentrations were evaluated in blood samples drawn 10 min post-DNP injection. **p < 0.01, versus WT and VVFF mice, Student t test.