FIG. 4.

Morphologically abnormal muscle fibers in arecoline-treated zebrafish embryos. (A) Lateral views of whole-mount immunofluorescence staining of slow myosin heavy chain in zebrafish embryos at 24, 48, 72, 96, and 120 hpf. The embryos are oriented in a cranial to caudal direction, from right to left. The whole mounts were viewed by confocal microscopy. Arrows indicate areas where twisted or broken fibers have been induced by the high concentration of arecoline (0.04%). Scale bar: 60 μm. (B) The number of slow muscle fibers per somite in the tail region between 24 and 120 hpf. (n=10). (*p<0.05). Color images available online at www.liebertpub.com/zeb