Skip to main content
. 2015 Feb 1;12(1):58–70. doi: 10.1089/zeb.2014.1010

FIG. 7.

FIG. 7.

Determination of the effect of arecoline on JC-1 staining patterns. The effect of arecoline on the loss of mitochondrial membrane potential in isolated mitochondria, as determined by JC-1 staining. The mean JC-1 fluorescence intensity was detected using fluorescence-activated cell sorting (FACS) analysis. A loss of mitochondrial membrane potential (ΔΨm) was demonstrated by the change in JC-1 fluorescence from red (JC-1 aggregates) to green (JC-1 monomers). (A) Analysis of mitochondrial membrane potential (ΔΨm) in each treatment group at 48 hpf. (B) The quantification of FACS analysis. The data are shown as the mean±SEM from three independent experiments. *p<0.01 versus control group. (C) The mitochondrial ultrastructure is shown in the tail region at 48 hpf. Representative transmission electron micrographs (×20,000).