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. 2015 Feb 1;12(1):33–47. doi: 10.1089/zeb.2014.0992

FIG. 3.

FIG. 3.

Effect of todralazine on recovery from chemically induced hemolytic anemia in Tg(gata1a: dsRed) zebrafish embryos. (a) Representative images of recovering embryos from PHZ-alone-treated embryos (iv, v at 96 and 120 hpf, respectively) or PHZ+todralazine treatments (vi, vii at 96 and 120 hpf, respectively). (i) Control embryos at 96 hpf and (ii, iii) are todralazine-alone-treated embryos at 96 and 120 hpf, respectively. Solid and dashed arrows points to intersegmental vessel (Se) and caudal artery, respectively. Arrowhead points to the brightly fluorescing erythroid progenitor population within the CHT region. (b) Quantitative real-time PCR analysis of expression of erythroid progenitor markers (αe1 and βe1) in wild-type zebrafish embryos. (c) A schematic representation of the experimental protocol of the gene expression analysis in the surgically separated tails of untreated or todralazine-treated wild-type zebrafish embryos. Asterisk (*) indicates p-value<0.05, when compared with control. CHT, caudal hematopoietic tissue; PCR, polymerase chain reaction.