FIG. 9.

(a) Effect of todralazine on radiation-induced apoptosis, measured in the notochord region or in whole embryos, 24 h after irradiation, by the acridine orange method. The integral density in the notochord region of different treatment groups was calculated using image J software. (b) Representative images showing acridine orange fluorescence in the notochord of the embryos. (c) Effect of todralazine on radiation-induced apoptosis measured in cell suspension prepared from the whole embryo 24 h after irradiation using flow cytometry and subG1 population was considered as apoptotic cells. Asterisk (*) indicates p-value<0.05, when comparison is done between indicated groups.