FIG. 4.
Effect of storage time and β93Cys on Prx-2 recovery after H2O2 exposure. RBCs from transgenic mice expressing human Hb with β93Cys or β93Ala were leukoreduced and stored as described in Materials and Methods section. After 1 or 10 days of storage, H2O2 (10 μM) was added and time-dependent (0–30 min) changes in Prx-2 oxidation were determined. (A) Prx-2 oxidation expressed as the fraction of the Prx-2 dimer relative to total Prx-2. Data are mean±SEM (n=4). *p<0.01 #p<0.05 compared with respective basal (time 0) by one-way RM-ANOVA with Tukey's post hoc test. **p<0.05 by t-test for comparison of maximal levels of Prx-2 oxidation at day 1 versus 10. (B) Time-dependent changes in Prx-2 relative to the maximal amount formed (at 5 min) with lines showing fits by linear regression. *p<0.01 between day 1 β93Cys and day 10 β93Cys RBC, #p<0.05 between day 1 β93Cys and day 1 β93Ala. (C) H2O2 concentrations (normalized to initial) after addition to β93Cys or β93Ala RBC stored for either 1 or 10 days. *p<0.05 relative to β93Ala RBC stored for 1 day by two-way RM-ANOVA with Bonferroni post hoc test (n=3–5). Hb, hemoglobin.