FIG. 5.

Effects of CO on Prx-2 recycling in β93Cys and β93Ala RBC. RBCs (stored for 1 day) were untreated, saturated with CO before, or 5 min after addition of H₂O₂ (10 μM), and time-dependent (0–30 min) changes in Prx-2 oxidation were determined as described in Materials and Methods section. (A, B) Time-dependent changes in Prx-2 relative to the maximal amount formed (at 5 min) for β93Cys and β93Ala RBC, respectively. Data are mean±SEM (n=3). *p<0.05 by two-way RM-ANOVA with Bonferroni post-test. (C) Effects of CO on H₂O₂-dependent Prx-2 oxidation relative to without CO in human RBC stored for 35 days. *p<0.05 by one-way RM-ANOVA relative to control. Data are mean±SEM, n=4. CO, carbon monoxide.