FIG. 7.

Formation of DHA at the β93 position of extracellular Hb during storage. Extracellular Hb was collected by centrifugation from human RBC stored for 7 or 35 days, and fate of the β93Cys was determined by tandem mass spectrometry. Shown is a representative mass spectrum (A), b and y ions from detected trypsin fragments; highlighted values indicate ions detected and non-highlighted values are predicted ions (B) and chromatogram depicting relative intensities of DHA in day 7 versus 35 sample (C). DHA, dehydroalanine. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars