Table 3.

Results and observations of growth experiments for Yanartaş enrichments.

Metabolic option	Growth conditions				Observations
	Temp. (°C)			pH
	30	40	50
Yeast and peptone (YP)	+++	+++	+++	8.5	Fastest growth at pH 8.3; reached cell densities of ~1.7 × 107 cells/ml within 4 h (at pH 8.3–9.5)
	++	++	++	9.5
	+	+	+	10.5
Organic acids (OA)	++	++	++	8.5	Lower cell density reached than with YP as a C source; cells appear shriveled in pH 10.4 media
	+	+	+	9.5
	+	+	+	10.5
Sugars (S)	+	+	N/A	8.5	Lower cell density reached than with YP or OA as a C source;
	+	+	N/A	9.5
	+	+	N/A	10.5
NO−3 reduction (NR)	++	++	++	8.5	Growth aided by addition of Corg
	+	+	+	9.5
	−	−	−	10.5
SO2−4 reduction (SR)	+	+	+	8.5	Growth aided by addition of Corg
	+	+	+	9.5
	−	−	−	10.5
Fe(III) red. (IR)	+	+	+	8.5	Growth aided by addition of Corg
	+	+	+	9.5
	−	−	−	10.5

All media formulations shown here were aerobic (anaerobic enrichments largely failed). The number of “+” signs indicates degree of growth success while “−” indicate no observed growth. A “+” indicates 10³–10⁴ cells ml⁻¹; “++” indicates 10⁵–10⁶ cells ml⁻¹; “+++”indicates 10⁷–10⁸ cell ml⁻¹.