Recipe 1:

BCDA (adapted from BCD; Cove et al., 2009c)

Reagent	Quantity (for 1 L)	Final concentration
Agar (Sigma-Aldrich A9799)	7 g	0.7% (w/v)
*CaCl2	111 mg	1 mM
FeSO4·7H2O	12.5 mg	45 μM
Solution B for moss media	10 mL	1 mM MgSO4
Solution C for moss media	10 mL	1.84 mM KH2PO4
Solution D for moss media	10 mL	10 mM KNO3
Hoagland’s A-Z trace element solution	1 mL	Trace
Diammonium tartrate	92 g	5 mM
*50 mg/mL vancomycin	1 mL	50 μg/mL

^*Do not add until after autoclaving.