Fig. 4.

Hypothetical BSA of the sh1 mutant in A619. The bulked samples are derived from an F2 mapping population with the inbred line B73. The represented SSRs (see Table 1) span the genome from bin 8.08 to bin 10.00. (1) wild-type parent, (2) mutant parent, (3) wild-type pool, (4) mutant pool, (5) F1. Based on the SSR patterns, there are two potentially linked SSRs, bnlg244 and umc1982. It is important to note, however, that only bnlg244 is flanked by SSR markers on adjacent bins with an overrepresentation of one of the two alleles. This is usually additional evidence for potential linkage. In the case of umc1982, the F1 sample suggests preferential amplification of one of the two segregating alleles (see Appendix 1, Note 10).