Table 1.

Simple sequence repeat (SSR) markers polymorphic between A619 and B73 for bulked segregant analysis (BSA). These SSR markers, ordered by chromosome (chr.) location, were selected uniquely based on gel patterns available at MaizeGDB. Note that for some bins no polymorphic SSR marker was available. By analyzing the bulk DNA samples with these markers, there is a good chance of obtaining a rough map location for the mutant of interest. We successfully mapped all five mutants that we attempted with this set. A similar list of polymorphic SSR markers useful for BSA in W22 × Mo17 and W22 × B73 mapping populations has been described (Martin et al., 2010).

chr.1	bin	chr.2	bin	chr.3	bin	chr.4	bin	chr.5	bin	chr.6	bin	chr.7	bin	chr.8	bin	chr.9	bin	chr.10	bin
bnlg1014	1.01	bnlg1338	2.01	umc2071	3.01	umc1232	4.00	umc1097	5.00	phi126	6.00	bnlg1367	7.00	umc1359	8.00	umc1279	9.00	umc1380	10.00
umc1222	1.02	bnlg1297	2.02	bnlg1144	3.02	umc2148	4.01	umc2036	5.01	bnlg1371	6.01	umc1409	7.01	umc1483	8.01	umc1040	9.01	umc1319	10.01
umc1880	1.03	bnlg2248	2.03	umc2158	3.04	umc1943	4.02	umc1587	5.02	umc1178	6.02	bnlg1247	7.02	umc1304	8.02	bnlg244	9.02	umc1337	10.02
bnlg2295	1.04	bnlg108	2.04	bnlg1505	3.05	umc2082	4.03	umc1935	5.03	umc1014	6.04	bnlg434	7.03	umc2146	8.03	umc1191	9.03	bnlg1037	10.03
umc1689	1.05	bnlg180	2.05	umc1949	3.06	bnlg490	4.04	bnlg1208	5.04	umc1805	6.05	bnlg1666	7.04	umc1172	8.04	bnlg1714	9.04	umc1053	10.04
umc1590	1.06	bnlg1396	2.06	umc1404	3.07	umc1303	4.05	bnlg1237	5.05	umc2170	6.06	umc1154	7.05	umc1562	8.05	umc1078	9.05	umc1506	10.05
umc1356	1.07	bnlg1045	2.07	umc1521	3.08	bnlg2291	4.06	umc1524	5.06	umc1248	6.07			umc1724	8.06	umc1310	9.06	bnlg1028	10.06
umc1991	1.08	bnlg1316	2.08	umc2152	3.09	umc1847	4.07	bnlg1346	5.07	umc1521	6.08			umc1268	8.07	bnlg128	9.07	bnlg1360	10.07
umc1082	1.09	bnlg1520	2.09	umc2048	3.10	umc1051	4.08	umc1225	5.08					umc1005	8.08	umc1982	9.08
bnlg1347	1.10					umc1101	4.09	umc1829	5.09					umc1663
bnlg1055	1.11					bnlg589	4.10							8.09
umc1797	1.12					bnlg2186	4.11