Figure 3.

Temporal expression profiles of key genes involved in regulating seasonal reproduction in the quail brain. A–C, Representative autoradiograms and densitometric quantitation are shown: TSHB in the pars tuberalis (A), DIO2 (B), and DIO3 (C) in the ependymal cells within the MBH. *, P < .05; **, P < .01 vs 0LD (n = 4–5, ANOVA). Solid line, SL; dashed line, SD. Note that in Figures 1–5, all samples were collected at 16 hours after dawn to examine the expressions of TSHB and DIO2 in the brain in the same animals. These genes are rhythmically expressed and show high expression levels at around this time of the day.