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. 2014 Nov 18;156(2):647–659. doi: 10.1210/en.2014-1741

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Figure 7. — Identification of the tissue responsible for low temperature-induced serum T₃. A and B, Changes in serum T₃ (A) and T₄ (B) levels under SD (dashed line) and SL (solid line) conditions. *, P < .05; **, P < .01 vs day 0. C and D, Changes in DIO2 mRNA in various tissues under SD (C) and SL (D) conditions. Representative autoradiograms (top panel) and densitometric quantification (bottom panel) are shown. **, P < .01 vs day 0. Scale bars, 1 cm. E, Size of thyroid gland did not change under SD and SL conditions. F, DIO2 activity in liver and testis under SD and SL conditions. Samples were collected 28 days after transferred to each condition. *, P < .05 (t test, n = 3–6). G, Castration did not affect low-temperature-induced serum T₃ level. *, P < .05; **, P < .01 day 0 vs day 20 (t test, n = 6–8). All samples were collected at 3 hours after dawn under SD/SL condition (ie, midday).